Signal Peptide Predictions

Protein secretion usually requires the presence of a N- terminal signal peptide (SP) and by scanning for this feature using available highly accurate SP-prediction tools, the fraction of potentially secreted proteins can be directly predicted. We were able to improve the in silico inventory of Aspergillus niger secretory proteins by combining different gene-model predictions from neighbouring Aspergilli and thereby avoiding prediction conflicts associated with inaccurate gene-models. An experimental ...

An experimental validation of the predicted proteome confirmed in silico predictions.

Secretome enriched fractions of A. niger N402 cultured under controlled conditions in defined synthetic media were analysed by high-throughput mass spectrometry