IBISBA Workshop

Protein secretion usually requires the presence of a N- terminal signal peptide (SP) and by scanning for this feature using available highly accurate SP-prediction tools, the fraction of potentially secreted proteins can be directly predicted. We were able to improve the in silico inventory of Aspergillus niger secretory proteins by combining different gene-model predictions from neighbouring Aspergilli and thereby avoiding prediction conflicts associated with inaccurate gene-models. An experimental ...

Protein secretion usually requires the presence of a N- terminal signal peptide (SP) and by scanning for this feature using available highly accurate SP-prediction tools, the fraction of potentially secreted proteins can be directly predicted. We were able to improve the in silico inventory of Aspergillus niger secretory proteins by combining different gene-model predictions from neighbouring Aspergilli and thereby avoiding prediction conflicts associated with inaccurate gene-models. An experimental ...

Test project

Protein secretion usually requires the presence of a N- terminal signal peptide (SP) and by scanning for this feature using available highly accurate SP-prediction tools, the fraction of potentially secreted proteins can be directly predicted. We were able to improve the in silico inventory of Aspergillus niger secretory proteins by combining different gene-model predictions from neighbouring Aspergilli and thereby avoiding prediction conflicts associated with inaccurate gene-models. An experimental ...

An experimental validation of the predicted proteome confirmed in silico predictions.

Investigation into optimizing micropollutant removal from wastewater treatment plant effluent using biological treatment and ozonation

What type of bioreactors are best at removing organic matter from wastewater treatment plant effluent. And what conditions can optimize these processes

16s Amplicon assay of sample BAC 09.16

16s Amplicon assay of sample BAC 09.16 sludge1

16s Amplicon assay of sample BAC 09.16 sludge2

16s Amplicon assay of sample BAC 09.17

16s Amplicon assay of sample BAC 09.18

Secretome enriched fractions of A. niger N402 cultured under controlled conditions in defined synthetic media were analysed by high-throughput mass spectrometry

Secretome enriched fractions of A. niger N402 cultured under controlled conditions in defined synthetic media were analysed by high-throughput mass spectrometry

demultiplexed reverse file

demultiplexed forward file

MIB Mock 4 community for postive amplicon sequencing control

MIB Mock 3 community for postive amplicon sequencing control

To get an idea of the background of microorganisms that is being fed to the reactors

We have been running three types of bioreactors (BAC, SF and MBBR) at different feed flow speeds (0.25 - 4 L / h) for several months, while monitoring several parameters related to the effluent organic matter (TOC, UV254 absorbance and COD), oxygen and nutrient concentrations in the water

We have been running three types of bioreactors (BAC, SF and MBBR) at different feed flow speeds (0.25 - 4 L / h) for several months, while monitoring several parameters related to the effluent organic matter (TOC, UV254 absorbance and COD), oxygen and nutrient concentrations in the water